Cell cultures have become an indispensable tool in the pharmaceutical industry and biomedical research, serving a wide range of applications from drug discovery to toxicity testing and vaccine development. This section outlines the various ways in which cell cultures are utilized in these fields, highlighting their significance and impact. 1. …
DeepakRajput 
Primary, Established, and Transformed Cell Cultures
Animal cell cultures are classified into three main categories based on their origin, lifespan, and characteristics: primary cultures, established (or continuous) cell lines, and transformed cell cultures. Each type serves unique purposes in research and biotechnology, and understanding their differences is crucial for selecting the appropriate culture for specific applications. …
Growth of Animal Cells in Culture
Animal cell culture is a fundamental technique in biological and medical research, enabling the study of cellular processes in a controlled environment. It involves the growth of cells derived from animal tissues under artificial conditions that simulate the natural environment. This technique is widely used in various fields, including drug …
Evaluation of Microbial Stability of Formulations
The evaluation of microbial stability in pharmaceutical formulations is crucial for ensuring product safety, efficacy, and shelf life. This process assesses a formulation’s ability to resist microbial contamination and growth over time, which is vital for products that are susceptible to spoilage, such as liquid formulations, creams, and ointments. Various …
Preservation of Pharmaceutical Products Using Antimicrobial Agents
The preservation of pharmaceutical products is essential to prevent microbial contamination, degradation, and spoilage, thereby ensuring their safety, efficacy, and shelf life. Antimicrobial agents, commonly referred to as preservatives, play a key role in protecting pharmaceutical products, especially those that contain water or are prone to contamination. These preservatives inhibit …
Sources and Types of Microbial Contaminants
Microbial contaminants can originate from various sources, impacting the safety and quality of pharmaceuticals, food products, and other environments. The primary sources of microbial contamination include the environment, raw materials, equipment, personnel, and packaging materials. 1. Environmental Sources: Air, water, and soil serve as major environmental reservoirs for microorganisms. Airborne …
Microbial Spoilage of Pharmaceutical Products: Definition, Types, and Factors Affecting Spoilage
Definition Microbial spoilage refers to the contamination and degradation of pharmaceutical products caused by the growth of microorganisms such as bacteria, fungi (molds and yeasts), and sometimes viruses. This spoilage can lead to a reduction in the quality, safety, and efficacy of the pharmaceutical product, rendering it unsuitable for use. …
Assessment of a New Antibiotic
The development and assessment of a new antibiotic involve several stages, including preclinical testing, clinical trials, and post-market surveillance. These stages ensure that the antibiotic is safe, effective, and has minimal resistance risk before and after its release to the market. 1. Preclinical Assessment This stage includes laboratory and animal …
Methods for Standardization of Antibiotics, Vitamins, and Amino Acids
The standardization of bioactive substances such as antibiotics, vitamins, and amino acids is crucial to ensure their potency, efficacy, and safety. Standardization is achieved through validated assays and procedures that determine the exact concentration, biological activity, and quality of these substances. Below are the commonly used methods for standardization: 1. …
Principles and Methods of Different Microbiological Assays
Microbiological assays are techniques used to estimate the concentration or potency of a substance (such as antibiotics, vitamins, or growth factors) by measuring its effect on a microorganism. These assays are essential in pharmacology, biotechnology, and microbiology for determining the activity of various substances in the presence of specific microbes. …
