Thin Layer Chromatography (TLC) is a widely used analytical technique that separates and identifies compounds in a mixture. It is based on the differences in the migration rate of compounds when carried by a mobile phase (solvent) over a stationary phase (adsorbent layer). Thin Layer Chromatography (TLC) is a type …
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Partition Column Chromatography: Adsorption chromatography: Definition, Principle, Methodology, Types and Applications
Partition column chromatography is a type of liquid-liquid chromatography where separation is based on the partitioning of components between two immiscible liquid phases—one as the stationary phase and the other as the mobile phase. This technique is commonly used for separating mixtures of compounds with varying solubilities and plays an …
Adsorption chromatography: Definition, Principle, Methodology, Types and Applications
Adsorption chromatography is a separation technique based on the differential adsorption of compounds onto a solid stationary phase. It is widely used to purify and separate chemical compounds based on their interaction with the adsorbent material. The process relies on the principle that different molecules have varying degrees of affinity …
Ion Exchange Chromatography (IEC): Introduction, Principle, Classification, Mechanism, Factor affecting, and Applications
Introduction Ion Exchange Chromatography (IEC) is a powerful and versatile separation technique primarily used to purify proteins, peptides, amino acids, and other charged biomolecules based on their ionic charge. It separates molecules by their affinity to charged ion-exchange resins in the stationary phase. This technique is widely used in the …
Gel Chromatography: Introduction, Theory, Instrumentation, and Applications
Gel chromatography, also known as size-exclusion chromatography (SEC), is a separation technique used primarily for separating molecules based on their size. It is widely used for purifying proteins, polysaccharides, and other macromolecules. In this technique, molecules are separated as they pass through a column filled with porous gel beads, and …
Affinity Chromatography: Introduction, Theory, Types, Instrumentation and applications
Introduction to Affinity Chromatography Affinity chromatography is a type of liquid chromatography used for the separation, purification, or specific analysis of sample components. It is based on the principle of affinity, which refers to reversible biological interactions or molecular recognition between two molecules, typically an analyte and a ligand. These …
