The absorption spectrum of a molecule represents how it absorbs light across various wavelengths. The environment of a molecule, particularly the solvent it is dissolved in, plays a crucial role in influencing its electronic transitions and, consequently, its absorption spectrum. The solvent can shift the absorption maxima, alter the intensity …
Spectral Shifts: Definition, Types, Factors, and Applications
Spectral shifts refer to changes in the position of the absorption or emission peaks of a molecule in a spectrum, often resulting from changes in the molecule’s environment, structure, or interactions. These shifts occur in response to variations in solvent polarity, pH, temperature, or the presence of substituents. Types of …
Auxochromes: Definition, Characteristics, Types, and Applications
An auxochrome is a functional group that does not itself absorb light in the visible spectrum but, when attached to a chromophore, modifies the chromophore’s ability to absorb light. It enhances the absorption intensity and often shifts the wavelength of maximum absorption (λmax)​of the chromophore. Characteristics of Auxochromes 1. Functional …
Electronic Transitions
Electronic transitions are the movements of electrons between energy levels (orbitals) in a molecule or atom when they absorb or emit energy, such as photons of light. These transitions form the basis of techniques like UV-visible spectroscopy and are critical in understanding the optical and electronic properties of materials. Types …
UV-Visible Spectroscopy: Introduction, Principle, Components, Applications
Introduction UV-Visible spectroscopy is an analytical technique that measures the absorption or reflectance of ultraviolet (UV) and visible light (200–800 nm) by a substance. It is widely used for quantitative and qualitative analysis of molecules in chemistry, biochemistry, and materials science. Principle of UV-Visible Spectroscopy The technique is based on …