Introduction
Enzymes are biological catalysts that accelerate chemical reactions in living organisms. Most enzymes are proteins, though some RNA molecules can also function as enzymes (ribozymes). They are essential for numerous biochemical processes, including digestion, metabolism, DNA replication, and cellular respiration. Enzymes work by lowering the activation energy of a reaction, thereby increasing the rate at which it occurs.
 Properties of Enzymes
1. Specificity: Enzymes are highly specific for their substrates. This specificity is often described by the “lock and key” model or the “induced fit” model, where the enzyme’s active site is precisely shaped to fit the substrate.
2. Catalytic Efficiency: Enzymes are incredibly efficient, often increasing reaction rates by factors of millions. They achieve this by stabilizing the transition state and decreasing the activation energy required for the reaction.
3. Saturation: Enzyme activity reaches a maximum rate at a certain substrate concentration, known as Vmax. This occurs because, at high substrate concentrations, all enzyme active sites are occupied.
4. Temperature and pH Sensitivity: Enzymes have optimal temperature and pH ranges in which they function most effectively. Outside these ranges, enzyme activity can decrease or the enzyme can become denatured and lose its functionality.
5. Reversibility: Most enzyme-catalyzed reactions are reversible, although the direction of the reaction depends on the concentrations of substrates and products.
6. Regulation: Enzymes are regulated by various mechanisms, including allosteric regulation, covalent modification, and changes in gene expression levels. This regulation ensures that enzyme activity is appropriate for the cell’s needs.
 Nomenclature of Enzymes
Enzyme names typically end in “-ase” and are often derived from the name of their substrate or the type of reaction they catalyze. For instance:
– Oxidoreductases: Enzymes that catalyze oxidation-reduction reactions.
– Transferases: Enzymes that transfer functional groups from one molecule to another.
– Hydrolases: Enzymes that catalyze the hydrolysis of various bonds.
– Lyases: Enzymes that break various chemical bonds by means other than hydrolysis and oxidation.
– Isomerases: Enzymes that catalyze the isomerization changes within a single molecule.
– Ligases: Enzymes that join two molecules with covalent bonds.
 IUBMB Classification of Enzymes
The International Union of Biochemistry and Molecular Biology (IUBMB) has developed a systematic method for classifying and naming enzymes known as the Enzyme Commission (EC) numbers. This system categorizes enzymes into six main classes based on the type of reaction they catalyze, each with further subclasses and sub-subclasses.
1. EC 1 – Oxidoreductases
– Catalyze oxidation-reduction reactions.
– Example: Alcohol dehydrogenase (EC 1.1.1.1)
2. EC 2 – Transferases
– Transfer functional groups between donor and acceptor molecules.
– Example: Alanine transaminase (EC 2.6.1.2)
3. EC 3 – Hydrolases
– Catalyze the hydrolysis of various bonds.
– Example: Lipase (EC 3.1.1.3)
4. EC 4 – Lyases
– Catalyze the addition or removal of groups to form double bonds.
– Example: Pyruvate decarboxylase (EC 4.1.1.1)
5. EC 5 – Isomerases
– Catalyze the rearrangement of atoms within a molecule.
– Example: Triose-phosphate isomerase (EC 5.3.1.1)
6. EC 6 – Ligases
– Catalyze the joining of two molecules with the use of ATP.
– Example: DNA ligase (EC 6.5.1.1)
Each enzyme is assigned a unique EC number consisting of four numbers separated by periods. The first number represents the main class, the second number the subclass, the third number the sub-subclass, and the fourth number the serial number of the enzyme in its sub-subclass.
Conclusion
Enzymes play a critical role in facilitating and regulating biochemical reactions in living organisms. Their specificity, efficiency, and regulation mechanisms make them indispensable for life. The systematic nomenclature and classification by IUBMB provide a structured way to identify and study the vast array of enzymes and their functions in biological processes.
