Electrophoresis: Introduction, methodology, development techniques, advantages, disadvantages and applications

Screenshot 2024 11 19 153138 Electrophoresis: Introduction, methodology, development techniques, advantages, disadvantages and applications

Introduction: Electrophoresis is a powerful analytical technique used to separate charged molecules, such as proteins, DNA, and RNA, based on their size, shape, and charge under the influence of an electric field. When an electric current is applied to a solution containing charged particles, these molecules move toward the electrode with the opposite charge. It … Read more

Paper Chromatography: Introduction, methodology, development techniques, advantages, disadvantages and applications.

Screenshot 2024 11 19 150411 Paper Chromatography: Introduction, methodology, development techniques, advantages, disadvantages and applications.

Introduction Paper chromatography is a simple and effective analytical technique used to separate and identify mixtures of substances. This method is classified under partition chromatography, where separation occurs based on the differential partitioning of solutes between a mobile phase (solvent) and a stationary phase (paper). It was first introduced in the early 20th century and … Read more

Thin Layer Chromatography (TLC): Definition, Principle, Methodology, Types and Applications 

Screenshot 2024 11 17 183245 Thin Layer Chromatography (TLC): Definition, Principle, Methodology, Types and Applications 

Thin Layer Chromatography (TLC) is a widely used analytical technique that separates and identifies compounds in a mixture. It is based on the differences in the migration rate of compounds when carried by a mobile phase (solvent) over a stationary phase (adsorbent layer). Thin Layer Chromatography (TLC) is a type of planar chromatography. It is … Read more

Partition Column Chromatography: Adsorption chromatography: Definition, Principle, Methodology, Types and Applications

Screenshot 2024 11 17 175118 Partition Column Chromatography: Adsorption chromatography: Definition, Principle, Methodology, Types and Applications

Partition column chromatography is a type of liquid-liquid chromatography where separation is based on the partitioning of components between two immiscible liquid phases—one as the stationary phase and the other as the mobile phase. This technique is commonly used for separating mixtures of compounds with varying solubilities and plays an important role in pharmaceutical, biochemical, … Read more

Adsorption chromatography: Definition, Principle, Methodology, Types and Applications

Screenshot 2024 11 17 171453 Adsorption chromatography: Definition, Principle, Methodology, Types and Applications

Adsorption chromatography is a separation technique based on the differential adsorption of compounds onto a solid stationary phase. It is widely used to purify and separate chemical compounds based on their interaction with the adsorbent material. The process relies on the principle that different molecules have varying degrees of affinity to the surface of a … Read more