Translation (Protein Synthesis): Definition, Steps, Inhibition, and Application

Translation is the process by which the genetic code carried by messenger RNA (mRNA) is decoded to produce a specific sequence of amino acids in a polypeptide chain, which folds into a functional protein. This process occurs in the cytoplasm of both prokaryotic and eukaryotic cells and involves ribosomes, transfer …

Genetic Code: Definition, Structure, and Codes

The genetic code is a set of rules by which the information encoded in genetic material (DNA or RNA sequences) is translated into proteins by living cells. Specifically, the genetic code defines how sequences of nucleotide triplets, called codons, specify which amino acids are added to a growing polypeptide chain …

Transcription (RNA Synthesis)

Transcription is the process by which genetic information encoded in DNA is copied into messenger RNA (mRNA). This is the first step in gene expression, leading to the synthesis of proteins. Transcription occurs in the nucleus of eukaryotic cells and in the cytoplasm of prokaryotic cells. Here is a detailed …

DNA Replication (Semi-Conservative Model)

DNA replication is a fundamental process by which a cell duplicates its DNA before cell division, ensuring that each daughter cell inherits an identical copy of the DNA. The semi-conservative model of DNA replication is the accepted mechanism by which DNA replication occurs. This model was proposed by Watson and …

Structure of DNA and RNA and Their Functions

DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) are nucleic acids essential for the storage, transmission, and expression of genetic information in all living organisms. Despite their similarities, they have distinct structures and functions.  Structure of DNA  Chemical Composition 1. Nucleotides:    – Components: Each nucleotide consists of three components:      …

Organization of the Mammalian Genome

The mammalian genome is a complex and highly organized structure composed of DNA and associated proteins, contained within the cell nucleus. This organization facilitates the efficient packaging, replication, and expression of genetic information. Here is a detailed note on the various aspects of mammalian genome organization:  Chromosomal Structure 1. Chromosomes: …

Catabolism of Purine Nucleotides

The catabolism of purine nucleotides involves the breakdown of adenine and guanine into uric acid, which is then excreted from the body. This process involves several enzymatic steps and occurs primarily in the liver.  Breakdown of Adenine Nucleotides 1. Deamination of AMP:    – Enzyme: AMP deaminase.    – Reaction: …

Biosynthesis of Purine and Pyrimidine Nucleotides

Nucleotide biosynthesis is a crucial biochemical process that generates the building blocks of DNA and RNA. It involves the synthesis of purine and pyrimidine nucleotides through complex pathways that ensure a balanced supply of these essential molecules for cellular functions. Purine Nucleotide Biosynthesis Purine nucleotides (adenine and guanine) are synthesized …

Nucleic Acid Metabolism

Nucleic acid metabolism encompasses the synthesis and degradation of nucleic acids, which include DNA and RNA. These processes are vital for the maintenance, replication, and expression of genetic information.  DNA Metabolism 1. DNA Replication:     – Initiation: Replication begins at specific locations in the genome called origins of replication. Initiator …

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