Therapeutic and Diagnostic Applications of Enzymes and Isoenzymes

 Introduction Enzymes and isoenzymes play crucial roles not only in metabolism but also in medical diagnostics and therapy. Enzymes can be utilized as drugs to treat specific diseases, while isoenzymes serve as important biomarkers for the diagnosis of various conditions. This note explores the therapeutic and diagnostic applications of enzymes …

Coenzymes: Structure and Biochemical Functions

 Introduction Coenzymes are organic non-protein molecules that bind to enzymes and assist in enzyme-catalyzed reactions. They often function as carriers of specific atoms or functional groups during the reaction. Coenzymes are crucial for the proper functioning of many enzymes and play vital roles in various metabolic pathways. Unlike enzymes, coenzymes …

Regulation of Enzymes: Enzyme Induction, Repression, and Allosteric Regulation

 Introduction The regulation of enzyme activity is critical for maintaining cellular homeostasis and responding to environmental changes. Enzymes are regulated through various mechanisms to ensure that metabolic pathways function efficiently and adaptively. This note covers enzyme induction and repression, as well as the regulation of allosteric enzymes.  Enzyme Induction and …

Enzyme Kinetics and Inhibition: Michaelis-Menten Plot, Lineweaver-Burk Plot, and Enzyme Inhibitors

 Enzyme Kinetics Enzyme kinetics is the study of the rates of enzyme-catalyzed reactions. It helps in understanding how enzymes work and how their activity can be modulated. Two important models in enzyme kinetics are the Michaelis-Menten model and the Lineweaver-Burk plot.  Michaelis-Menten Kinetics The Michaelis-Menten equation describes the rate of …

Enzymes: Introduction, Properties, Nomenclature, and IUBMB Classification

 Introduction Enzymes are biological catalysts that accelerate chemical reactions in living organisms. Most enzymes are proteins, though some RNA molecules can also function as enzymes (ribozymes). They are essential for numerous biochemical processes, including digestion, metabolism, DNA replication, and cellular respiration. Enzymes work by lowering the activation energy of a …

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