Introduction Paper chromatography is a simple and effective analytical technique used to separate and identify mixtures of substances. This method is classified under partition chromatography, where separation occurs based on the differential partitioning of solutes between a mobile phase (solvent) and a stationary phase (paper). It was first introduced in …
Qualitative tests and functions of lipids
1. Sudan III Staining The Sudan III staining is a laboratory technique used to detect the presence of lipids, particularly triglycerides and fats, in biological samples. Sudan III is a lipophilic dye, meaning it has an affinity for lipids and can bind to them, resulting in a visible color change. …