Thin-Layer Chromatography Archives

Identification Tests of Volatile Oils

November 18, 2023 by DeepakRajput

1. Organoleptic Evaluation Principle: Assessment of sensory properties, including aroma and flavor, through smell and taste. Application: Initial qualitative evaluation of essential oil characteristics. 2. Physical Characteristics Color and Appearance: Principle: Visual inspection for color variations and overall appearance. Application: Helps identify impurities or alterations in the essential oil. Specific Gravity: Principle: Determination of the … Read more

Identification of Glycosides

November 17, 2023 by DeepakRajput

Identifying glycosides involves a series of tests and analytical techniques to determine these compounds’ presence, structure, and characteristics. Here are some common methods used for the identification of glycosides: 1. Chemical Tests Legal’s Test:Observe the reaction mixture for any color change. The development of a brown-to-black color characterizes a positive result. Molisch’s Test: A positive … Read more

Identification of terpenoids

November 16, 2023 by DeepakRajput

The identification of terpenoids involves a series of tests and analytical techniques to determine these compounds’ structural characteristics and specific properties. Here are some common identification tests for terpenoids: 1. Physical Properties Odor and Color: Terpenoids often have distinctive odors and colors. Observation of these properties can provide preliminary information. 2. Thin-Layer Chromatography (TLC) Principle: … Read more

Identification of alkaloids

November 16, 2023November 16, 2023 by DeepakRajput

Identifying alkaloids is crucial in studying these naturally occurring compounds, allowing researchers to determine their presence and classify them based on structural and chemical characteristics. Various methods and tests are employed for the identification of alkaloids. Here is a detailed note on the identification of alkaloids: 1. Physical Characteristics a. Appearance: Alkaloids often have … Read more

Alkaloids – Definition, Isolation, Occurrence

November 16, 2023 by DeepakRajput

Alkaloids are a group of naturally occurring organic compounds, predominantly of plant origin, characterized by their basic nitrogen-containing structures. These compounds often exhibit pronounced physiological effects on humans and other animals. Alkaloids are known for their pharmacological activities, including analgesic, stimulant, sedative, and toxic properties. Common examples of alkaloids include morphine, caffeine, quinine, and nicotine. … Read more